Unsere Kollegin Ludivine Hugoni vom Labor INSERM Biomaterials and Bioengineering präsentierte am Donnerstag, den 30. Mai 2019, während des Termis EU 2019 Kongresses in Rhodos die Ergebnisse des Nanotransmed Projektes.
Poly(arginine) grafted to biphosphonated dendrons: a novel strategy to design antimicrobial coatings for biomaterial applications
- Hugoni1,2, D. V. Nguyen3, N. Zydziak4, F. Boulmedais4, D. Felder-Flesch3, P. Lavalle1,2
Presenting Author: Ludivine Hugoni,
1INSERM UMR 1121, Strasbourg, France; 2Université de Strasbourg, Faculté́ de Chirurgie Dentaire, Strasbourg, France; 3CNRS UMR 7504, Strasbourg, France; 4CNRS UPR 22 Strasbourg, France
INTRODUCTION: After the introduction of biomaterials into the body, the surface of implants acts as an optimal substrate for bacterial growth, which increases the risk of developing infections. To modulate bacterial-surface interactions and prevent subsequent infections leading to the implant failure, one strategy consists in designing bioactive coatings on the surfaces. Herein, biphosphonated dendrons (BP‑D) were deposited covalently on oxide surfaces. The BP‑D presented specific -COOH end groups, which enable to easily functionalize the surfaces with antimicrobial biomolecules such as poly(arginine) (PAR) [1, 2]. Hence, promising coatings for antimicrobial applications were designed here.
METHODS: Prior to PAR functionalization, BP‑D (1 mg/mL) were coated on the surface of SiO2 sensors and the deposition was analyzed by Quartz Crystal Microbalance (QCM). PAR10 (1 mg/mL) was then grafted through EDC/NHS chemistry. To characterize the grafting on the BP‑D coated surfaces, PAR10‑FITC was used and the mean intensity of fluorescence was analyzed by fluorescence microscopy (FM), after 24 h incubation in RPMI 1640 medium at 37 °C. Staphylococcus aureus suspension was deposited on the coated surfaces and incubated for 24 h at 37°C. Bacteria adhered to the surfaces were stained using the BacLight RedoxSensor CTC Vitality Kit, fixed with 4 % PFA to be further observed by FM.
RESULTS: Through QCM analysis, optimal conditions for BP‑D deposition on surfaces were determined (data not shown). The grafting of PAR10‑FITC on BP‑D was then checked by FM: higher intensity of fluorescence was observed for the peptide covalently grafted than adsorbed. After the incubation of S. aureus on the coated surfaces, results indicated that only PAR10 grafted on BP‑D presented a huge decrease of bacterial adhesion.
Figure 1: A. Mean intensity of fluorescence for PAR10-FITC adsorbed vs grafted B. S. aureus growth on the coated surfaces (all bacteria: green; active bacteria: red)
DISCUSSION & CONCLUSIONS: Taken together, these results showed the efficacy of PAR10 covalently grafted to BP‑D for antimicrobial coating. This constitutes a suitable strategy to functionalize surfaces and contribute to the success of implantable devices.
ACKNOWLEDGEMENTS: This study is co-funded through the project Nanotransmed by the European Regional Development Fund in the framework of the INTERREG V Upper Rhine program (Transcending borders with every project) & by the Swiss Confederation, the swiss cantons of Aargau, Basel-Landschaft and Basel-Stadt.
 Özçelik et al. P. Adv. Healthc. Mater. 2015 4 2026-2036  Mutschler et al. Chem. Matter. 2017 29 3195–3201